Vision Research Protocols

Product Details
Book Publisher: Humana Press (15 January, 2001)
ISBN: 0896038378
Book author: P. Elizabeth Rakoczy
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Elizabeth Rakcozy and a team of leading clinical and experimental scientists describe in step-by-step detail the key techniques essential to effective molecular biological research in ophthalmology and optometry. These readily reproducible methods are adapted to the special requirements of vision research, with coverage that ranges from the most basic to the most sophisticated technologies. Included are methods for the down-regulation of gene expression, new gene therapy techniques, and for the development of transgenic and knockout animal models for testing novel therapies. Eminently accessible and clinically relevant, Vision Research Protocols provides experimental and biomedical investigators in ophthalmology and optometry with a rich panoply of most powerful tools with which to ask–and answer–all the important questions emerging from the dramatically advancing work in vision research today.

1. Basic Molecular Biology Techniques
Chooi-May Lai
pp. 1-30

2. Transient Transfection of Human Retinoblastoma Cells: Application to the Analysis of the Regulatory Regions of Photoreceptor-Specific Genes
Debora Farber; Leonid Lerner; Andrea Viczian
pp. 31-43

3. In Situ Hybridization
Xian-Jie Yang
pp. 45-69

4. Immunocytochemical Studies of the Retina
Ann Milam
pp. 71-88

5. Antisense DNA Technology
Piroska Rakoczy
pp. 89-104

6. Inhibition of Gene Expression by Ribozymes William W. Hauswirth, Lynn C. Shaw, Patrick O. Whalen, Jason J. Fritz,
D. White; Alfred Lewin
pp. 105-124

7. Intraocular Delivery of Recombinant Virus
Fong-Qi Liang; Vibha Anand; Albert Maguire; Jean Bennett
pp. 125-139

8. Preparation of Recombinant Adenoviruses
Karl Csaky
pp. 141-155

9. Generation of Recombinant Adeno-Associated Virus
Fabienne Rolling
pp. 157-170

10. Preparation of Recombinant Retroviruses

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