Hardcover: 185 pages
Publisher: Humana Press; 1 edition (July 19, 2004)
Language: English
ISBN-10: 0896039498
ISBN-13: 978-0896039490
File type : PDF
File size : 3.8 MB
Book Description
Expert laboratorians detail emerging methodologies for studying the cell cycle, its kinases, and kinase inhibitors. The authors focus the issues of gene expression in vivo and in vitro, the analysis of cyclin-dependent kinase inhibitors, protein degradation mediated by the proteosome, the analysis of the transformed cell phenotype, and innovative techniques to detect apoptosis. Many of the protocols are new, such as electron microsocopy to detect apoptosis and proteosome-mediated degradation, whereas others study the interactions between protein-protein, protein-DNA, and protein-RNA.