http://www.book4doc.com/16451
* Publisher: Humana Press
* Number Of Pages: 376
* Publication Date: 2005-08-15
* Sales Rank: 1507317
* ISBN / ASIN: 1588294420
* EAN: 9781588294425
* Binding: Hardcover
* Manufacturer: Humana Press
* Studio: Humana Press
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Book Description:
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This extensively updated and expanded second edition contains new chapters on the elctrophysiological measurement of Ca2+ channel activity, enhanced coverage of confocal microscopy, and practical tutorials on two of the most common industrial high-throughput machines, the Fluormetric Imaging Plate Reader (FLIPR), and the FlexStation. All experimental chapters provide readily reproducible techniques for measuring the many dacets of Ca2+ signaling. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.